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目的探讨异功散对炎症诱发的铁代谢紊乱的影响。方法 40只C57BL/6小鼠随机分为空白对照组、异功散组、脂多糖组、异功散加脂多糖组,每组10只。异功散组、异功散加脂多糖组给予异功散灌胃10.57 g/(kg·d),空白对照组每只灌胃0.2 ml超纯水,各组均连续灌胃7天。第8天,脂多糖组和异功散加脂多糖组腹腔注射脂多糖1.5 mg/kg建立铁代谢紊乱模型,6h后处死所有小鼠。检测血常规、血清铁和肝组织铁含量、血清和肝组织白细胞介素6(IL-6)含量。结果各组小鼠血常规无明显变化,差异无统计学意义(P>0.05)。与空白对照组比较,脂多糖组小鼠血清铁降低,肝组织铁和血清、肝组织IL-6均明显上升(P<0.05),而异功散组加脂多糖组较脂多糖组肝组织铁、血清和肝组织IL-6均明显下降(P<0.05)。结论异功散可改善脂多糖介导的急性炎症条件下的铁代谢紊乱,其机制可能与降低血清和肝组织IL-6有关。
Objective To investigate the effect of Yigong San on inflammation-induced iron metabolism disorders. Methods Forty C57BL / 6 mice were randomly divided into control group, Ischemic group, lipopolysaccharide group and Isoproterenol plus lipopolysaccharide group, with 10 mice in each group. Heterofacial powder group and Heterofacial powder plus lipopolysaccharide group were given intragastric 10.57 g / (kg · d), while the blank control group was fed with 0.2 ml of ultrapure water per group. Each group was given continuous gavage for 7 days. On the 8th day, the model of iron metabolism was established by intraperitoneal injection of lipopolysaccharide (1.5 mg / kg) into the lipopolysaccharide group and the dyslipidemia plus lipopolysaccharide group, and all the mice were killed 6 hours later. The levels of iron, serum iron and hepatic tissue iron, interleukin 6 (IL-6) in serum and liver tissue were detected. Results There was no significant difference in blood routine between the three groups (P> 0.05). Compared with the blank control group, the levels of serum iron in the lipopolysaccharide-treated mice decreased significantly (P <0.05), whereas the levels of IL-6 in the iron, serum and liver of the LPS group were significantly higher than those in the LPS group IL-6 in iron, serum and liver tissue were significantly decreased (P <0.05). Conclusion IWG can ameliorate the disorder of iron metabolism induced by lipopolysaccharide in acute inflammation, which may be related to the reduction of IL-6 in serum and liver tissue.