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作者对31例再生障碍性贫血患者进行了淋巴细胞亚群及淋巴细胞功能测定。31例中14例为重型再障。男15例,女16例,平均年龄39±19.2岁。11例在初次诊断时进行检查,20例在诊断后不同病期进行检查,以年龄及性别相当的100例正常人作为对照组。测定方法:取外周血,用Ficoll-Hypaque分离得单个核细胞(MNC),调细胞浓度为1×10~6/ml,作以下检查。淋巴细胞亚群:用纯化的单克隆抗体Leu1、Leu2、Leu3、OKIal,以间接荧光法测定。
The author of 31 cases of aplastic anemia in patients with lymphocyte subsets and lymphocyte function determination. Fourteen of 31 cases were aplastic aneurysms. There were 15 males and 16 females, with an average age of 39 ± 19.2 years. Eleven cases were examined at initial diagnosis, 20 cases were examined at different stages after diagnosis, and 100 normal subjects of the same age and gender as control group. Determination: Take peripheral blood mononuclear cells (MNC) were isolated with Ficoll-Hypaque, adjusted cell concentration of 1 × 10 ~ 6 / ml, for the following examination. Lymphocyte subsets: Measured by indirect fluorescence with the purified monoclonal antibodies Leu1, Leu2, Leu3, OKIal.