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目的探讨组织microRNA-21表达、高危型人类乳头瘤病毒(HPV)载量与宫颈癌发生发展的相关性。方法选取2012年2月至2015年8月间东莞市东城医院接诊的150例宫颈病变并行宫颈活检患者。根据病理检测结果分为观察组(120例)和对照组(30例)。观察组为宫颈癌检测呈现宫颈疾病的患者,对照组为无宫颈疾病者。分析microRNA-21在患者组织细胞中的表达,宫颈疾病与HPV感染率、HPV病毒载量中位数和HPV病毒载量的关系。结果 150例患者中,宫颈病变明显者占80.0%。观察组患者microRNA-21表达显著高于对照组,差异有统计学意义(P<0.05)。伴随着病变程度的加重,HPV感染率也呈现逐步上升的趋势,高度病变患者的HPV感染率达93.8%,患者的HPV感染阳性与阴性比较差异有统计学意义(P<0.05)。宫颈癌患者的HPV病毒载量最高,其次是宫颈上皮内瘤样病变(CIN),CIN中伴随着病变严重程度的加剧,HPV病毒载量也呈现上升的趋势,CIN的HPV病毒载量又显著高于慢性宫颈炎,伴随宫颈疾病的病变程度不同,其HPV病毒载量的分布情况也大不相同。结论 MicroRNA-21在宫颈癌细胞中表达存在异常,HPV病毒载量是严重影响宫颈病危险因素之一,宫颈癌的严重程度和临床分期与HPV病毒载量呈正相关关系。病毒载量越高,宫颈癌的分化程度越低,临床分期就会越高。
Objective To investigate the relationship between microRNA-21 expression, high-risk human papillomavirus (HPV) load and the development of cervical cancer. Methods From February 2012 to August 2015, 150 patients with cervical lesions who underwent cervical biopsy in Dongguan Dongcheng Hospital were enrolled. According to the pathological examination results were divided into observation group (120 cases) and control group (30 cases). The observation group was cervical cancer patients with cervical cancer detection, the control group without cervical disease. To analyze the expression of microRNA-21 in human tissue cells, the relationship between cervical diseases and HPV infection, the median HPV viral load and HPV viral load. Results 150 cases of patients, cervical lesions were 80.0%. The expression of microRNA-21 in observation group was significantly higher than that in control group (P <0.05). With the increasing severity of the disease, the HPV infection rate also showed a gradual upward trend. The HPV infection rate was 93.8% in patients with highly pathological changes, and there was significant difference between the positive and negative HPV infection patients (P <0.05). Cervical cancer patients with the highest HPV viral load, followed by cervical intraepithelial neoplasia (CIN), CIN with the severity of the disease intensified, HPV viral load also showed an upward trend, CIN HPV viral load was significant Higher than chronic cervicitis, accompanied by varying degrees of cervical disease lesions, the distribution of HPV viral load is also very different. Conclusion The expression of MicroRNA-21 in cervical cancer cells is abnormal. The HPV viral load is one of the risk factors for cervical cancer. The severity and clinical stage of cervical cancer are positively correlated with HPV viral load. The higher the viral load, the lower the degree of cervical cancer differentiation, the clinical stage will be higher.