论文部分内容阅读
目的确定天山岩黄芪中总黄酮的优选超声提取工艺。方法采用正交实验法,考察溶剂量,提取次数,超声时间,溶剂浓度对提取率的影响,以芦丁为标准品,用紫外分光光度标准曲线法测定含量。结果所考察的因素中,天山岩黄芪总黄酮的提取影响程度为:溶剂浓度>提取时间>溶剂量>提取次数,即乙醇浓度70%,提取时间30 min,体积为40 ml,提取次数为3次。结论超声乙醇提取法最佳工艺组合A3B3C3D3,可为利用天山岩黄芪工业生产黄酮类药用成分提供科学依据。
Objective To determine the optimal ultrasonic extraction process of total flavonoids in Astragalus membranaceus. Methods Orthogonal experiment was used to determine the effects of solvent amount, extraction times, ultrasonic time and solvent concentration on the extraction rate. Rutin was used as the standard and the content was determined by UV spectrophotometric standard curve method. Results Among the investigated factors, the extraction degree of total flavonoids from Astragalus membranaceus was as follows: solvent concentration> extraction time> solvent amount> extraction times, ie ethanol concentration 70%, extraction time 30 min, volume 40 ml, extraction times 3 Times. Conclusion Ultrasonic ethanol extraction combined with A3B3C3D3 can provide the scientific basis for the industrial production of flavonoids in T. astragalus.