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目的建立广藿香油中广藿香酮的高效液相色谱测定法。方法采用高效液相色谱法。色谱柱为YMC-Pack C18色谱柱(250×4.6 mm,5μm);流动相:乙腈-0.2%磷酸溶液(60∶40,V/V);检测波长:310 nm;流速:1 mL.min-1;柱温:35℃。结果广藿香酮在0.25~5μg范围内呈良好线性关系,r=1.0000,平均回收率为101.16%,RSD=1.49%(n=6);10批样品中广藿香酮含量在3.54～40.19 mg.g-1范围内。结论此法简便快速、准确可靠,可用于广藿香油含量的测定和质量监控。
Objective To establish a HPLC method for the determination of patchoulenone in patchouli oil. Methods Using high performance liquid chromatography. The chromatographic column was a YMC-Pack C18 column (250 × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.2% phosphoric acid solution (60:40 V / V), the detection wavelength was 310 nm, the flow rate was 1 mL.min- 1; column temperature: 35 ℃. Results There was a good linear relationship between patchoulenone in the range of 0.25 ~ 5μg, r = 1.0000, the average recovery was 101.16%, RSD = 1.49% (n = 6). The patchoungone content of 10 batches of samples was between 3.54 and 40.19 mg.g-1 range. Conclusion This method is simple and rapid, accurate and reliable, and can be used for the determination and quality control of patchouli oil.