论文部分内容阅读
目的探讨不同浓度维甲酸对神经干细胞的增殖和分化的效应。方法分离、培养胎龄14d的Wistar孕鼠的神经干细胞,通过与碱性成纤维细胞生长因子(bFGF)对比观察维甲酸的促增殖效应;运用神经微丝200(NF-200)和神经胶质酸性纤维蛋白(GFAP)对维甲酸诱导分化的细胞进行组化染色,通过4’,6-二脒基-2-苯基吲哚(DAPI)染胞核,计数各种分化细胞的比例,并对分化的神经元进行胆碱能表型鉴定。结果维甲酸的增殖效应明显弱于bFGF,但其具有明显的促神经元生成的分化效应,并表达递质,在分化第24天,500nmol/L的浓度使分化的神经元占分化的总细胞数的90.80%。结论维甲酸具有显著的促神经干细胞分化成神经元的效应。
Objective To investigate the effects of different concentrations of retinoic acid on the proliferation and differentiation of neural stem cells. Methods Neural stem cells of pregnant Wistar pregnant mice were isolated and cultured for 14 days. Contrasting with basic fibroblast growth factor (bFGF), the proliferative effects of retinoic acid were observed. The effects of retinoic acid 200 (NF-200) and glia Acid fibrin (GFAP) was stained for retinoic acid-induced differentiated cells and the proportion of various differentiated cells was counted by staining nuclei with 4 ’, 6-diamidino-2-phenylindole (DAPI) Cholinergic phenotype identification of differentiated neurons. Results The proliferative effect of retinoic acid was weaker than that of bFGF. However, it had obvious differentiating effect on neurogenesis and expression of neurotransmitter. On the 24th day of differentiation, the concentration of 500 nmol / L made the differentiated neurons account for the differentiated total cells 90.80% of the number. Conclusion Retinoic acid has the remarkable effect of differentiating neural stem cells into neurons.