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目的研究补充不同剂量、不同接触时间的维生素E对致敏SD大鼠机体敏感性的影响。方法取8周龄雌性SD大鼠,按体重随机分为5组,每组25只,每天经口分别给予不同剂量的维生素E(2.3、6.9、13.8mg/kg),在第2周、3周、4周、5周、6周时,每组分别取出5只动物,腹腔注射给予10 mg/只OVA致敏,一周后使用50 mg/只OVA刺激,检测各组动物血清中总Ig E、特异性Ig E和Ig G1、血浆中组胺、m MCP-1和细胞因子(IFN-γ,IL-4)的水平,大刺激后处死动物,取心脏、肝、脾、肾、胸腺、空肠、肠系膜淋巴结等脏器,并对这些脏器进行组织病理学观察。结果经口给予高剂量维生素E 6周,OVA产生的免疫学反应强度受到了显著的抑制作用,总Ig E、特异性Ig E、特异性Ig G1、IL-4以及组胺水平显著降低,IFN-γ水平显著升高,随接触时间的延长呈增高的趋势。且高剂量处理组动物免疫学反应强度显著低于OVA对照处理组,对致敏SD大鼠的机体敏感性起到了有效的调节作用。结论维生素E能够降低致敏动物机体对卵白蛋白的敏感性,也抑制了免疫系统对卵白蛋白产生的免疫学反应。
Objective To study the effects of vitamin E supplemented with different doses and different exposure times on the sensitization of sensitized SD rats. Methods 8-week-old female Sprague-Dawley rats were randomly divided into 5 groups with 25 rats in each group. Different doses of vitamin E (2.3, 6.9, 13.8 mg / kg) 5 weeks and 6 weeks, 5 animals in each group were taken out, intraperitoneal injection of 10 mg / OVA sensitized, once a week after stimulation with 50 mg / OVA, the total serum IgE , Specific Ig E and Ig G1, plasma histamine, m MCP-1 and cytokines (IFN-γ, IL-4) Jejunum, mesenteric lymph nodes and other organs, and histopathological observation of these organs. Results After 6 weeks of oral administration of high-dose vitamin E, the immunological reaction intensity of OVA was significantly inhibited. The levels of total Ig E, specific Ig E, specific Ig G1, IL-4 and histamine were significantly decreased. IFN -γ levels were significantly increased, with the extension of exposure time showed an upward trend. The immunological reaction intensity of high dose treatment group was significantly lower than that of OVA control group, which effectively regulated the body sensitivities of sensitized SD rats. Conclusion Vitamin E can reduce the sensitivities of sensitized animals to ovalbumin and inhibit the immunological response of the immune system to ovalbumin.