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Objective:To design a software to do the complicated and multiple calcula-tions automatically in routine internal radionuclide irradiation therapy to avoid mistakes and shorten patients waiting times. Methods:The software is designed on the Microsoft Windows XP operating system. Visual Basic 5.0 and Microsoft Access 2000 are used re-spectively as the programming language and database system here. The data and DBGrid controls and VB data window guide of Visual Basic were used to control access to and Ac-cess database. Results: Not only can the radioactivity of any radionuclide be calculated, but also the administered total iodine dose of therapy for hyperthyroidism or thyroid cancer and the total administered 153 Sm-EDTMP solutions for remedy of bone metastasis of malig-nant tumor can be ciphered out. Conclusion: The work becomes easier, faster, more cor-rect and interesting when the software can make the complicated and multiple calculations automatically. Patients information, diagnosis and treatment can be recorded for further study.