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B淋巴细胞表面具有多种标志,可用不同的方法予以检测。诸如检测B淋巴细胞表面免疫球蛋白(SIg)的免疫萤光法、测定Fc受体的EA花环、以及检测C_3受体的EAC花环试验、FBC花环试验、酵母补体花环试验等,从不同的角度反映了B细胞的存在及其数量。由于不同种属动物的免疫反应功能不尽相同,为了选择理想的动物模型。根据我们的实验条件采用了比较简易的酵母补体花环法,研究了影响方法准确性的各种因素,并测定了人和六种哺乳类动物的外周血B淋巴细胞正常值,为深入研究免疫反应提供参数。
B lymphocytes with a variety of signs on the surface can be detected using different methods. Such as immunofluorescence assay on B lymphocyte surface immunoglobulin (SIg), determination of Fc rosette on EA receptor, EAC garland assay for detecting C_3 receptor, FBC rosette assay, yeast complement rosette assay, etc. From different perspectives Reflects the presence and amount of B cells. Due to different immune functions of different species of animals vary, in order to choose the ideal animal model. Based on our experimental conditions, a relatively simple yeast complement rosette method was used to study various factors that affect the accuracy of the method. The normal levels of B lymphocytes in peripheral blood of humans and six mammalian species were determined. In order to further study the immune response Provide parameters.