以蚂蚱腿子幼嫩带芽茎段为外植体,通过对其芽启动诱导、继代增殖及生根诱导培养基的筛选,建立了蚂蚱腿子的离体快速繁殖技术体系。结果表明:最适腋芽诱导培养基为B_5+1.0 mg·L~(-1)6-BA+0.1 mg·L~(-1)IBA,外植体诱导萌发率达80.0%;最适增殖培养基为B_5+1.0 mg·L~(-1)6-BA+0.3 mg·L~(-1)IBA,增殖系数可达7.6;最佳生根培养基为1/2 B_5+0.1 mg·L~(-1)IBA+0.1 mg·L~(-1)NAA,生根率可达93.3%,平均主根数量达11.2条。炼苗后,移栽于珍珠岩和蛭石(1:1,V/V)或腐殖土和河沙(2:1,V/V)混合基质中。成活率均达90%以上。 The young grasshopper leg with bud stem segments as explants, through its bud induction, subculture and rooting medium selection, the establishment of the grasshopper leg in vitro rapid propagation technology system. The results showed that the most suitable induction medium for axillary buds was B 5 + 1.0 mg · L -1 6-BA + 0.1 mg · L -1 IBA, and the induction rate of germination was 80.0% The proliferation coefficient was up to 7.6 for the B_5 + 1.0 mg · L -1 (-1) 6-BA + 0.3 mg · L -1 IBA. The best rooting medium was 1/2 B_5 + 0.1 mg · L ~ (-1) IBA + 0.1 mg · L -1 NAA, the rooting rate reached 93.3% and the average number of main roots reached 11.2. The seedlings were transplanted into a mixed matrix of perlite and vermiculite (1: 1, V / V) or humus and sand (2: 1, V / V). Survival rate reached more than 90%.