目的对与唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌有关的4株重要的菌株进行表型性状描述,以证明它们是同一个菌种。方法着重用传统的底物利用试验和GC及专用软件(Sherlock系统6.1)分析菌细胞的脂肪酸,做全面的表型描述。结果 4株菌的30项生化反应完全一致,170项底物利用试验95%一致,12种脂肪酸及脂肪酸组合基本一致;4株菌都是固氮菌。结论 4个株菌为同一个菌种,都可称为唐菖蒲伯克霍尔德氏菌。 Objective To characterize four important strains associated with Burkholderia gladioli and Burkholderia coconut and demonstrate that they are one and the same species. The method focuses on the analysis of fatty acids in bacterial cells with traditional substrate utilization tests and GC and proprietary software (Sherlock System 6.1) for a complete phenotypic description. Results The results showed that 30 biochemical reactions of the four strains were identical, 95% of 170 substrates were identical, and 12 kinds of fatty acids and fatty acid compositions were basically the same. All four strains were nitrogen-fixing bacteria. Conclusion Four strains of the same strain, can be called Burkholderia gladius.