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目的:筛选出具有促进骨骼肌细胞L6表面GLUT4转位活性的委陵菜黄酮衍生物。方法:将实验细胞分成空白对照组,胰岛素组(以胰岛素刺激20 min)和黄酮衍生物组(加不同衍生物刺激24 h),用类ELISA法测定细胞膜上GLUT4的量。结果:对黄酮衍生物1~14影响L6大鼠骨骼肌细胞GLUT4转运的实验结果表明,衍生物1、5、6、8~11在10μg/mL作用浓度下,促进GLUT4转位的量分别为空白对照组的(3.60±0.30)倍、(3.66±0.26)倍、(2.87±0.49)倍、(3.97±0.37)倍、(2.82±0.45)倍、(3.37±0.67)倍、(4.43±0.61)倍(P<0.05)。化合物6与胰岛素叠加作用为空白对照组的(4.31±0.22)倍(P<0.05)。结论:委陵菜黄酮衍生物促进GLUT4转位的作用为首次报道。黄酮衍生物1、5、6、8~11有明显地促进L6表面GLUT4转位的作用;化合物6与胰岛素有协同作用。
OBJECTIVE: To screen for the extract of Cephalosporium flavonoids with GLUT4 translocation activity on L6 surface of skeletal muscle cells. Methods: The experimental cells were divided into blank control group, insulin group (stimulated by insulin for 20 min) and flavone derivative group (stimulated by different derivatives for 24 h). The amount of GLUT4 on the cell membrane was determined by ELISA. Results: The results of GLUT4 transport in skeletal muscle cells of L6 rat induced by flavonoid derivatives 1-14 showed that the amount of GLUT4 translocation promoted by derivatives 1, 5, 6, 8-11 at the concentration of 10μg / mL was (3.60 ± 0.30) times, (3.66 ± 0.26) times, (2.87 ± 0.49) times, (3.97 ± 0.37) times, (2.82 ± 0.45) times, (3.37 ± 0.67) times, (4.43 ± 0.61) times of the blank control group ) Times (P <0.05). Compound 6 and insulin superposition of the blank control group (4.31 ± 0.22) times (P <0.05). CONCLUSION: The first report about the role of the extract of C. phyllanthyllum in promoting GLUT4 translocation was first reported. Flavone derivatives 1, 5, 6 and 8 ~ 11 obviously promoted the translocation of GLUT4 on L6 surface; Compound 6 had synergistic effect with insulin.