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目的比较胰岛素强化治疗前后口服抗糖尿病药失效2型糖尿病(T2DM)患者外周血单核细胞中核因子κB(NF-κB)表达水平的变化,探讨胰岛素的抗炎作用。方法选择对照组20名(NC组),口服药失效的T2DM患者20例(DM组)给予胰岛素强化治疗2周。检测DM组治疗前后空腹血糖(FBG)、空腹胰岛素(Fins)和c肽以及餐后2hBG、胰岛素和c肽水平的变化及采用流式细胞仪(FCM)、竞争性酶联免疫吸附法(ELISA)检测治疗前后外周血单核细胞NF-κB表达水平的变化。结果与NC组比较,T2DM患者外周血单核细胞NF-κB表达升高(P<0.01);与治疗前比较,胰岛素强化治疗2周后,NF-κB表达降低(P<0.01);FBG、2hBG和HOMA-IR降低;胰岛素、C肽和HOMA-β升高。结论 T2DM患者2周胰岛素强化治疗后,血糖明显改善的同时,单核细胞中NF-κB表达明显降低。
Objective To compare the changes of nuclear factor κB (NF-κB) in peripheral blood mononuclear cells of patients with type 2 diabetes mellitus (T2DM) before and after intensive insulin treatment before and after intensive insulin treatment and to explore the anti-inflammatory effect of insulin. Methods Twenty control subjects (NC group) and 20 T2DM patients (DM group) were given insulin intensive therapy for 2 weeks. The changes of fasting blood glucose (FBG), fasting insulin (Fins) and c-peptide, 2-hour post-prandial blood glucose, insulin and c-peptide levels in DM group before and after treatment were detected by flow cytometry (FCM) and competitive enzyme-linked immunosorbent assay ) To detect the changes of NF-κB in peripheral blood mononuclear cells before and after treatment. Results Compared with NC group, the expression of NF-κB in peripheral blood mononuclear cells was significantly increased in T2DM patients (P <0.01). Compared with NC group, the expression of NF-κB decreased after 2 weeks of intensive insulin treatment (P <0.01) 2hBG and HOMA-IR decreased; insulin, C-peptide and HOMA-β increased. Conclusion After 2 weeks’ intensive insulin therapy, the blood glucose is significantly improved in T2DM patients, and the expression of NF-κB in mononuclear cells is significantly decreased.