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目的 研究类风湿关节炎 (RA)共同表位QK/RRAA及其变异对细胞内蛋白激酶A(PKA)信号通路的影响。方法 测定HLA DRβ1 0 40 4及其变异体转基因细胞内PKA、cAMP及腺苷环化酶 (AC)的浓度及活性。结果 HLA DRβ1 0 40 4转基因细胞的PKA活力、cAMP水平及AC活力明显低于HLA DRβ1 0 40 3转基因细胞株 (P <0 0 1)。HLA DRβ1 0 40 4序列第 70～ 74位氨基酸QRRAA中的Q70 及A74 是抑制PKA信号通路的关键氨基酸。结论 类风湿关节炎共同表位QK/RRAA的表达可抑制PKA、cAMP和AC的活性 ,并由此参与类风湿关节炎的发病
Objective To investigate the effect of QK / RRAA and its mutations on the protein kinase A (PKA) signaling pathway in patients with rheumatoid arthritis (RA). Methods The concentration and activity of PKA, cAMP and adenosyl cyclase (AC) in the HLA DRβ1404 and its mutants were determined. Results PKA activity, cAMP level and AC activity of HLA DRβ1 0 40 4 transgenic cells were significantly lower than that of HLA DRβ1 0 40 3 transgenic cell line (P <0.01). HLA DRβ1 0 40 4 Sequence Amino Acids 70-74 Q70 and A74 in QRRAA are the key amino acids that inhibit PKA signaling. Conclusions The expression of QK / RRAA, a common epitope of rheumatoid arthritis, inhibits the activity of PKA, cAMP and AC, and thus participates in the pathogenesis of rheumatoid arthritis