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目的研究血管紧张素转换酶抑制剂(ACEI)中的福辛普利(fosinopril)、卡托普利(captopril)及I型血管紧张素II受体拮抗剂(ARB)中缬沙坦(valsartan)对人血单核细胞受细菌脂多糖(LPS)刺激表达组织因子(TF)的影响。方法从健康平产妇脐静脉取得脐血,分离单个核细胞,分组培养,以LPS(01mg/L)刺激作为对照组,其它组分别再加入ACEI类药物fosinopril(20mg/L)、captopril(20mg/L)及valsartan(20mg/L),孵育。冻融法收集细胞裂解液,用一期凝固法分析LPS诱导单核细胞表达的组织因子促凝活性。用RT-PCR技术,观察各组表达TFmRNA,并以GAPDH作为内参照进行半定量分析。结果和结论Fosinopril,captopril及valsartan可下调人血单核细胞由LPS诱导的TFmRNA的表达,并显著降低促凝活性。
AIM To investigate the effects of valsartan in fosinopril, captopril and type I angiotensin II receptor antagonist (ARB) in angiotensin-converting enzyme inhibitor (ACEI) On human blood mononuclear cells stimulated by bacterial lipopolysaccharide (LPS) to stimulate the expression of tissue factor (TF). Methods Umbilical cord blood was harvested from healthy uterine umbilical vein. Mononuclear cells were isolated and cultured in groups. LPS (01mg / L) was used as the control group. The other groups were given ACEI drugs fosinopril (20mg / L), captopril L) and valsartan (20 mg / L) and incubated. Cell lysate was collected by freeze-thaw method. Tissue factor procoagulant activity of LPS-induced monocytes was analyzed by one-stage coagulation assay. The expression of TF mRNA in each group was observed by RT-PCR and semi-quantitative analysis was performed with GAPDH as internal reference. RESULTS AND CONCLUSION Fosinopril, captopril and valsartan down-regulated the expression of TFmRNA induced by LPS in human blood mononuclear cells and significantly reduced procoagulant activity.