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目的:检测Ndrg2在培养大鼠神经干细胞(NSCs)中的表达情况,探索改变Ndrg2表达对NSCs增殖和分化的影响。方法:用免疫细胞化学染色和Western Blot法检测Ndrg2在培养NSCs中的表达;利用AAV-Ndrg2过表达病毒及LV-Ndrg2-RNAi干扰病毒感染大鼠NSCs后,通过BrdU掺入实验观察干预Ndrg2表达后NSCs增殖的变化,利用神经元标记物Tuj1和星型胶质细胞标记物GFAP的免疫细胞化学染色分析NSCs的分化情况。结果:Ndrg2高表达于大鼠NSCs中;与对照组(感染病毒空载体)相比,上调Ndrg2表达可显著增加BrdU~+和Tuj1~+细胞数(P<0.05),但GFAP~+细胞数目无明显变化(P>0.05);相反,下调Ndrg2表达可减少BrdU~+细胞数(P<0.05),但不影响Tuj1~+和GFAP~+细胞数(P>0.05)。结论:Ndrg2表达于大鼠NSCs,它可正性调控NSCs的增殖并促进NSCs向神经元分化。
OBJECTIVE: To detect the expression of Ndrg2 in cultured rat neural stem cells (NSCs) and explore the effect of Ndrg2 expression on the proliferation and differentiation of NSCs. Methods: The expression of Ndrg2 in NSCs was detected by immunocytochemical staining and Western Blot. After infected with AAV-Ndrg2 overexpression virus and LV-Ndrg2-RNAi, the expression of Ndrg2 was detected by BrdU incorporation assay After NSCs proliferated, the differentiation of NSCs was analyzed by immunocytochemical staining of neuronal markers Tuj1 and astrocyte markers GFAP. Results: Ndrg2 was highly expressed in NSCs. Compared with the control group (empty vector), up-regulation of Ndrg2 significantly increased the number of BrdU ~ + and Tuj1 ~ + cells (P <0.05), but the number of GFAP ~ + cells On the contrary, down-regulation of Ndrg2 could decrease the number of BrdU + cells (P <0.05), but did not affect the numbers of Tuj1 + and GFAP + cells (P> 0.05). Conclusion: Ndrg2 is expressed in rat NSCs. It can positively regulate the proliferation of NSCs and promote the differentiation of NSCs into neurons.