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稻米种皮颜色可以分为白、红、黑等。在红色稻米种皮中富含原花青素,其具有抗氧化活性,有利于人体健康。本研究以419份广西地方稻种资源核心种质为实验材料,利用全基因组关联分析验证了控制水稻红色种皮的基因Rc。利用In Del标记HM1对97份红色种皮材料的DNA进行PCR,发现C323和C139属于第7外显子有14 bp缺失的红色种皮品种。然后,我们利用Sanger测序技术对这两个自然突变品种的Rc基因进行外显子测序,分析结果表明Rc ~(C139)和Rc ~(C323)属于2种不同的Rc等位基因类型。
Rice seed coat color can be divided into white, red, black and so on. The red rice seed coat is rich in proanthocyanidins, which have antioxidant activity, is conducive to human health. In this study, 419 core collections of rice germplasms in Guangxi were used as experimental materials, and genome-wide association analysis was used to verify the gene Rc controlling rice red seed coat. PCR was performed on the DNA of 97 red seed coat materials using In Del-labeled HM1. C323 and C139 belonged to the red seed coat with 14 bp deletion in exon 7. Then, we sequenced the Rc gene of these two naturally mutated varieties by Sanger sequencing. The results showed that Rc ~ (C139) and Rc ~ (C323) belong to two different Rc alleles.