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目的:探讨桂产藿香蓟乙醇提取物的抗炎作用及其作用机制。方法:采用二甲苯诱导小鼠耳廓肿胀,观察桂产藿香蓟乙醇提取物对二甲苯诱导小鼠耳廓肿胀的影响;采用角叉菜胶致小鼠足肿胀法,观察桂产藿香蓟乙醇提取物对角叉菜胶致小鼠足肿胀的影响,并分别测定小鼠炎性组织中丙二醛(MDA)、前列腺素E2(PGE2)和超氧化物歧化酶(SOD)的活性;采用角叉菜胶致大鼠足肿胀法,观察桂产藿香蓟乙醇提取物对角叉菜胶诱导大鼠足肿胀的影响,同时测定大鼠血清肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)和白介素-6(IL-6)的含量。结果:与空白对照组比较,桂产藿香蓟乙醇提取物能显著抑制小鼠耳廓肿胀及足肿胀(P<0.05或0.01),其中高、中、低剂量组(6.0,3.0,1.5 g·kg-1)耳廓肿胀和足肿胀抑制率分别为29.24%,16.42%,11.21%和28.66%,18.79%,13.13%,并能降低小鼠炎足中炎性组织PGE2、MDA含量,提高其SOD活力(P<0.05或0.01);显著抑制大鼠的足肿胀(P<0.05或0.01),其中在3 h时高、中、低剂量组(6.0,3.0,1.5 g·kg-1)的足肿胀抑制率分别为43.69%,36.01%,23.29%,并能显著降低肿胀足大鼠血清TNF-α、IL-1β和IL-6的含量(P<0.05或0.01)。结论:桂产藿香蓟乙醇提取物抗炎作用显著,其机制可能与清除氧自由基、减少炎症因子和致炎细胞因子的释放有关。
Objective: To investigate the anti-inflammatory effect and mechanism of alcohol extract of Ageratum asarum. Methods: The auricle swelling of mice was induced by xylene. The effects of Ethanolic extract of Ageratum inoculation on the auricle swelling induced by xylene in mice were observed. The carrageenan- The effect of thymus alcohol extract on carrageenan-induced paw edema in mice and the activity of malondialdehyde (MDA), prostaglandin E2 (PGE2) and superoxide dismutase (SOD) The carrageenan-induced rat paw edema was used to observe the effect of ethanol extract of Ageratum ascendensii on carrageenan-induced rat paw edema. Serum tumor necrosis factor (TNF-α), interleukin -1β (IL-1β) and interleukin-6 (IL-6). Results: Compared with the blank control group, Ethanol extract of Ageratum ashweed significantly inhibited auricle swelling and foot swelling in mice (P <0.05 or 0.01), and high, medium and low dose groups (6.0, 3.0 and 1.5 g · Kg-1), the inhibitory rates of auricle swelling and foot swelling were 29.24%, 16.42%, 11.21% and 28.66%, 18.79% and 13.13%, respectively. The inhibitory rates of PGE2 and MDA in inflammatory tissues of mice (P <0.05 or 0.01), and significantly inhibited foot swelling in rats (P <0.05 or 0.01). At 3 h, high, medium and low dose groups (6.0, 3.0 and 1.5 g · kg -1) (P <0.05 or 0.01). The levels of TNF-α, IL-1β and IL-6 in the rats with swelling were significantly lower than those in the control group (P <0.05 or 0.01). Conclusion: The anti-inflammatory effect of alcohol extract of Ageratum asiatica is remarkable. The mechanism may be related to scavenging oxygen free radicals and reducing the release of inflammatory cytokines and inflammatory cytokines.