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目的观察1,25-(OH)2D3及复方丹参对异基因骨髓移植(Allo-BMT)后大鼠T细胞亚群和细胞因子的影响。方法以雄性SD大鼠为供鼠,雌性Wistar大鼠为受鼠,40只受鼠随机分成aGVHD组及干预组,干预组包括1,25-(OH)2D3组、复方丹参组、两药联合组,每组10只。观察各组T细胞亚群(CD4+、CD8+)和细胞因子(IL-2、IFN-γ、IL-4、IL-10)的变化。结果当aGVHD表现较明显时,CD4+、CD8+增高,以CD4+增高为主,与移植前比差异有统计学意义(P<0.05);经干预方案处理后CD4+、CD8+增高的幅度较aGVHD组低,差异有统计学意义(P<0.05),以CD4+更为明显;动态监测显示与Thl相关的细胞因子IL-2、IFN-γ在各干预组呈下降趋势,与Th2相关的细胞因子IL-10呈上升趋势,均在第21天变化较为明显,与第0天相比有统计学差异(P<0.05);IL-4无显著性变化。结论1,25-(OH)2D3及复方丹参能够降低CD4+T淋巴细胞的数量,具有调节Thl/Th2平衡,抑制Thl的增殖及功能发挥,促进Th2细胞增殖,发挥免疫耐受的作用。
Objective To observe the effect of 1,25-(OH)2D3 and compound salvia miltiorrhiza on T lymphocyte subsets and cytokines after allo-BMT. Methods Male SD rats were used as donors, female Wistar rats were as recipients, and 40 rats were randomly divided into aGVHD group and intervention group. The intervention group consisted of 1,25-(OH)2D3 group, compound Danshen group and two drugs combination. Groups, 10 per group. Changes in T cell subsets (CD4+, CD8+) and cytokines (IL-2, IFN-γ, IL-4, IL-10) were observed in each group. Results When the aGVHD performance was obvious, the CD4+ and CD8+ levels increased, and the CD4+ level increased. There was a significant difference from the pre-transplantation ratio (P<0.05). The CD4+ and CD8+ levels after the intervention program treatment were lower than those in the aGVHD group. The difference was statistically significant (P<0.05), with CD4+ being more apparent; dynamic monitoring showed that Thl-related cytokines IL-2 and IFN-γ showed a decreasing trend in each intervention group, and Th2-related cytokine IL-10 The upward trend showed a significant change on the 21st day, and there was a statistically significant difference from the 0th day (P<0.05). There was no significant change in IL-4. Conclusion 1,25-(OH)2D3 and compound salvia miltiorrhiza can reduce the number of CD4+ T lymphocytes, regulate the Th1/Th2 balance, inhibit the proliferation and function of Th1, promote the proliferation of Th2 cells, and play a role in immune tolerance.