应用电泳技术分析了两个甘蓝型双低杂交油菜Ｆ１及三系的酯酶同工酶，结果表明，杂种与不育系、恢复系的酶谱存在差异，杂种Ｆ１的酶谱兼有两亲本的特征酶带，为从遗传上证实Ｆ１存在杂种优势提供了理论依据；研究还发现油菜酯酶同工酯酶谱与品种类型有密切关系。因此，选择适宜的生长时期，利用酯酶同工酶谱鉴定这两个组合的杂种及其纯度是可行的． The results of electrophoresis showed that there was a difference in the zymogram of F1 and three lines between two hybrids, Which provided a theoretical basis for confirming the existence of heterosis in F1. The study also found that the esterase isozyme pattern of rapeseed is closely related to the variety type. Therefore, the selection of suitable growth period, the use of esterase isozyme identification of these two combinations of hybrids and its purity is feasible.