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<正> 该研究建立一种快速、灵敏的方法,通过使用弓形虫特异性荧光猝灭探针,以real-time PCR法来检测和定量弓形虫模板DNA。此探针是通过引物设计软件设计出的一段靶基因反义链特异性的序列,含有35个核苷酸。其原理是:探针首先与弓形虫特异性引物扩增出的靶序列进行内杂交,然后
TaqManPCR定量检测人类体液中的弓形虫DNA
【摘 要】
：
<正> 该研究建立一种快速、灵敏的方法,通过使用弓形虫特异性荧光猝灭探针,以real-time PCR法来检测和定量弓形虫模板DNA。此探针是通过引物设计软件设计出的一段靶基因反义
【出 处】
：
国外医学：寄生虫病分册
【发表日期】
：
2002年2期
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