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目的检测可溶性肿瘤坏死因子相关的凋亡诱导配体(sTRAIL)在老年慢性阻塞性肺疾病(COPD)患者外周血的表达,探讨其与疾病严重程度的相关性。方法采用酶联免疫吸附试验(ELISA)法检测25例COPD急性加重期患者、25例COPD稳定期患者及30名健康对照外周血sTRAIL及肿瘤坏死因子-α(TNF-α)水平,同时采用速率散射比浊法检测血清C反应蛋白(CRP)浓度,分析sTRAIL与TNF-α、CRP的相关性。结果COPD急性加重期患者外周血sTRAIL、TNF-α及CRP明显高于COPD稳定期及健康对照组(P均<0.05);COPD稳定期患者sTRAIL及TNF-α明显高于健康对照组(P<0.05),而CRP与健康对照组间差异无统计学意义。COPD急性加重期患者血清sTRAIL与TNF-α、CRP呈显著正相关。结论老年COPD患者血清sTRAIL升高,并且急性加重期与TNF-α、CRP呈显著相关性,提示sTRAIL可能参与COPD患者病理损伤,且与疾病严重程度密切相关。
Objective To detect the expression of soluble tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) in peripheral blood of elderly patients with chronic obstructive pulmonary disease (COPD) and to explore its relationship with the severity of the disease. Methods The levels of sTRAIL and tumor necrosis factor-α (TNF-α) were measured in 25 patients with COPD exacerbation, 25 patients with stable COPD and 30 healthy controls by enzyme-linked immunosorbent assay (ELISA) Serum C-reactive protein (CRP) levels were measured by nephelometry. The correlation between sTRAIL and TNF-α and CRP was analyzed. Results The levels of sTRAIL, TNF-α and CRP in peripheral blood of COPD patients were significantly higher than those of healthy controls (P <0.05). The levels of sTRAIL and TNF-α in stable COPD patients were significantly higher than those in healthy controls (P < 0.05), but no significant difference between CRP and healthy control group. Serum sTRAIL in patients with acute exacerbation of COPD was positively correlated with TNF-α and CRP. Conclusion Serum sTRAIL levels are elevated in elderly patients with COPD, and there is a significant correlation between TNF-α and CRP in acute exacerbations. It suggests that sTRAIL may be involved in the pathological changes in patients with COPD and is closely related to the severity of the disease.