论文部分内容阅读
将序列超速离心结合凝胶层析分离纯化的Ｌｐ（ａ）进行Ｌ－Ｌｙｓｉｎｅ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析，分离出能与柱结合的Ｌｐ（ａ）Ｌｙｓ－和不能与柱结合的Ｌｐ（ａ）Ｌｙｓ－；进一步观察了功能异质性的上述两种Ｌｐ（ａ）对纤溶酶原与血小板结合的影响，结果表明：Ｌｐ（ａ）Ｌｙｓ＋能有效地抑制纤溶酶原与血小板的结合，其ＩＣ５０约为０．１９ｍｇ／ｍＬ，而Ｌｐ（ａ）Ｌｙｓ－则无明显抑制作用。
The Lp (a) Lys- and Lp (a) which can not bind to the column were isolated by L-Lysine-Sepharose 4B affinity chromatography after ultracentrifugation and gel filtration. The effect of Lp (a) on plasminogen binding to platelets was further observed. The results showed that Lp (a) Lys + could effectively inhibit the binding of plasminogen to platelets, The IC50 was about 0.19mg / mL, while Lp (a) Lys- had no significant inhibitory effect.