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采用体外抗氧化活性(包括DPPH自由基清除法、ABTS自由基清除法和FRAP法)为导向,筛选红花抗氧化活性部位,随后对活性部位进行成分分离与抗氧化效应评价。从红花活性部位水部位中分离得到5个成分,分别鉴定为6-羟基山柰酚-3,6,7-三-O-β-D-葡萄糖苷(1)、6-羟基山柰酚-3-O-β-芸香糖苷-6-O-β-D-葡萄糖苷(2)、6-羟基山柰酚-3-O-β-D-葡萄糖苷(3)、羟基红花黄色素A(4)和脱水红花黄色素B(5)。通过对红花不同极性部位和单体成分进行抗氧化效应比较,发现水部位的抗氧化活性较显著,从中分离得到6-羟基山柰酚糖苷类和醌式查尔酮碳苷类主要活性物质。
Antioxidant activity (including DPPH radical scavenging assay, ABTS radical scavenging assay and FRAP assay) was used as a guide to screen anti-oxidant active site of safflower, then the active site was separated and the anti-oxidant effect was evaluated. Five components were isolated from the water part of safflower flower and identified as 6-hydroxy-kaempferol-3,6,7-tri-O-β-D-glucoside (1) -3-O-β-rutinoside-6-O-β-D-glucoside (2), 6-hydroxy behenol-3-O- β-D-glucoside (3) A (4) and Dehydrated safflor yellow B (5). By comparing the antioxidative effects of different polar sites and monomer components of safflower, we found that the antioxidant activity of water fraction is more significant, and the main activities of 6-hydroxy-kaempferol and quinone-type chalcone are isolated substance.