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目的探讨邻苯二甲酸丁苄酯(butyl benzyl phthalate,BBP)暴露对仔代大鼠发育的影响。方法将80只4周龄Wistar雌性大鼠按体重随机分为4组,每组20只,分别喂饲BBP含量为0、0.05%、0.25%和0.75%(分别相当于每日50、250和750 mg/kg体重)的饲料,从4周龄持续到其仔鼠出生后第21天(postnatal day 21,PND21)断乳结束(包括雌性大鼠交配期、孕期及哺乳期);仔鼠断乳前与母鼠同笼饲养,断乳后至PND49,仔鼠按性别分笼饲养,喂饲方式和染毒剂量与其亲代母鼠相同。观察母鼠的一般情况,记录其妊娠时间、产仔数,计算分娩率、仔鼠性别比等;测定不同日龄仔鼠的体重以及脑、心、肝、脾、肺、肾、睾丸(或子宫、卵巢)重量,并计算脏器系数;测定仔鼠血清相关生化指标水平。结果与对照组比较,250 mg/kg组雌雄仔鼠脾脏系数升高(P<0.05),750 mg/kg组雌雄仔鼠肝脏系数升高(P<0.01),雄性仔鼠睾丸系数升高(P<0.05);各剂量BBP染毒组母鼠的分娩率、妊娠时间、产仔数、仔鼠性别比无明显变化;250、750 mg/kg组雄性仔鼠血清丙氨酸氨基转移酶(ALT)活力均明显升高;各剂量染毒组血清总胆红素(TBIL)含量均下降;750 mg/kg组血清总胆固醇(CHO)含量和碱性磷酸酶(ALP)、胆碱酯酶(CHE)活力均升高;雌性大鼠仅血清TBIL含量下降(P<0.05);其他指标未见异常。结论 BBP暴露影响仔鼠的肝脏、脾脏及睾丸发育,BBP致雄性仔鼠肝功能损伤较雌性仔鼠严重。
Objective To investigate the effects of butyl benzyl phthalate (BBP) exposure on the development of offspring rats. Methods Eighty four-week-old Wistar female rats were randomly divided into 4 groups according to body weight: 20 rats in each group, with BBP levels of 0,0.05%, 0.25% and 0.75% respectively (equivalent to 50,250 and 750 mg / kg body weight) from 4 weeks of age until the end of postnatal day 21 (PND21) at the end of weaning (including female rats mating, pregnancy and lactation) Pre-and post-maternal females were kept in cages, weaning to PND49, pups were housed by sex and cage, feeding method and exposure dose were the same as those of their mothers. The pregnant rats were observed for their general condition, the time of their pregnancy, the number of litter size, the rate of delivery and the sex ratio of the pups were recorded. The body weight, brain, heart, liver, spleen, lung, kidney and testis Uterus, ovary) weight, and calculate the organ coefficient; determination of serum related biochemical indicators of offspring. Results Compared with the control group, the spleen coefficient of male and female offspring in 250 mg / kg group increased (P <0.05), the liver coefficient of male and female 750 mg / kg group increased (P <0.01) P <0.05). There was no significant change in the delivery rate, gestational time, litter size and the sex ratio of offspring in BBP exposure group. Serum alanine aminotransferase ALT). The levels of total bilirubin (TBIL) in each dose group were decreased. The levels of total cholesterol (CHO), alkaline phosphatase (ALP), cholinesterase (CHE) activity increased; female rats only serum TBIL content decreased (P <0.05); other indicators no abnormalities. Conclusion BBP exposure affects the development of liver, spleen and testis in the offspring. The BBP-induced male rats’ liver damage is more serious than that of the female offspring.