金针菇是我国人工栽培发展较快的食用菌品种,为了加快发展盐城市金针菇生产,筛选出适合本市气候条件的优良菌株,我们于1994年至1996年,进行了本项研究,现将结果报告如下: 1 材料和方法 1.1 供试菌种 从曲阜、无锡、三明、成都、扬州引进8903(金83)、9201(金91)、苏金7号(金7)、苏金8号(金8)、三明1号(金1)、杂交19(金19)、8801(金81)、四川12号(金12)、金40共9个菌株。 1.2 菌种制作 母种采用综合马铃薯加富培养基,无菌操作接种,置23℃下培养观察菌丝长势。原种用棉壳培养基,棉壳80％,米糠17％,糖1％,多元素石膏1.5％,磷铵0.5％,含水量63％,pH6.8。750mL蘑菇瓶装湿料450g,1.5kg/cm~2灭菌70分钟,无菌操作接种,每支25×200mL试管母种转接6瓶原种,置25、29、32℃下培养观察其发菌情况。 1.3 栽培方法 500mL罐头瓶熟料栽培,栽培料配方:棉壳80％,玉米粉5％,米糠12％,多元素石膏2％,磷铵0.5％,石灰粉0.5％,含水量64％,pH7.0。每瓶装湿料400g(折干重144g),1.5kg/cm~2灭菌90分钟,无菌操作接种,每瓶原种接70瓶,在暗室里发好 In order to speed up the production of Flammulina velutipes in Yancheng City, Flammulina velutipes is a kind of edible fungus which has been cultivated rapidly in our country. The best strains suitable for the climatic conditions of this Municipality were screened out. From 1994 to 1996, this study was carried out and the results were reported As follows: 1 Materials and methods 1.1 The test species from the introduction of Qufu, Wuxi, Sanming, Chengdu, Yangzhou 8903 (gold 83), 9201 (gold 91), Sukin 7 (gold 7), Sujin 8 ), Sanming 1 (gold 1), hybrid 19 (gold 19), 8801 (gold 81), Sichuan 12 (gold 12), gold 40 a total of 9 strains. 1.2 The production of the mother species using integrated potato rich media, aseptic operation inoculation, set at 23 ℃ culture observed mycelial growth. The original species cotton shell culture medium, cotton shell 80%, rice bran 17%, sugar 1%, 1.5% multi-element gypsum, 0.5% ammonium phosphate, water content 63%, pH6.8.750mL Mushroom bottled wet material 450g, 1.5kg / cm ~ 2 sterilization 70 minutes, inoculation aseptic operation, each 25 × 200mL test tube mother transfer 6 bottles of original species, set 25,29,32 ° C under the observation of its strain of bacteria. 1.3 cultivation methods 500mL canned bottle of clinker cultivation, planting material formula: cotton shell 80%, corn flour 5%, 12% rice bran, 2% multi-element gypsum, 0.5% ammonium phosphate, lime powder 0.5%, water content 64% .0. Each bottle of wet material 400g (fold dry weight 144g), 1.5kg / cm ~ 2 sterilization 90 minutes, sterile operation inoculation, each bottle of 70 bottles of seed, made in the dark room