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利用紫外吸收和荧光光谱法研究了不同钌(Ⅱ)配合物[Ru(bpy)2MDHIP]2+(MDHIP=2,4-二羟基-咪唑并[4,5-f]][1,10]邻菲咯啉)和[Ru(bpy)2ODHIP]2+(ODHIP=3,4-二羟基-咪唑并[4,5-f]][1,10]邻菲咯啉)与DNA的作用机制,并研究了这两个配合物与Cu2+的配位情况及配位形成双核配合物后的荧光性质变化.结果表明取代基的位置对配合物与DNA的作用机制及荧光性质等都具有较大影响.配体MDHIP上的2-位OH可以与相邻咪唑环上的N原子形成分子内氢键,增强配合物与DNA的插入作用.但其4-位OH取代基又会产生一定的位阻,使配合物只能以部分插入模式与DNA作用.而配体ODHIP上3-和4-位的两个OH取代基不能与相邻咪唑环上的N原子形成分子内氢键,并会产生更大的空间位阻,使得配合物不能插入到DNA的碱基对中.但ODHIP上的2个OH可以同时与Cu2+配位形成双核配合物,使配合物与DNA的作用模式由沟面结合改为插入结合,配合物的荧光减弱.
The effects of different Ru (Ⅱ) complexes [Ru (bpy) 2MDHIP] 2+ (MDHIP = 2,4-dihydroxyimidazo [4,5-f] Phenanthroline and the mechanism of action of [Ru (bpy) 2ODHIP] 2+ (ODHIP = 3,4-dihydroxy-imidazo [4,5-f]] [1,10] , And studied the coordination between the two complexes and Cu2 + and the fluorescence properties of the complex after the formation of dinuclear complex.The results show that the position of the substituent has a greater effect on the mechanism of action and fluorescence properties of the complex with DNA Effect. The 2-position OH on the ligand MDHIP can form an intramolecular hydrogen bond with the N atom on the adjacent imidazole ring to enhance the insertion of the complex and DNA, but the 4-position OH substituent will produce a certain number of positions So that the complex can only interact with the DNA in a partial insertion mode while the two OH substituents at the 3- and 4-positions of the ligand ODHIP can not form intramolecular hydrogen bonds with N atoms on the adjacent imidazole ring and Resulting in a larger steric hindrance, so that the complex can not be inserted into the DNA base pairs.But ODHIP 2 OH at the same time can be coordinated with Cu2 + to form a dinuclear complex, the complex and DNA mode of action by the groove surface Combination with insert into the combination, with Fluorescence was weakening.