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利用差速离心法从葡萄果肉组织中分离了微粒体和胞质组分，将微粒体进一步利用两相分配法进行了质膜和细胞内膜的分离。结果表明，微粒体分离是整个实验技术的难点；研磨缓冲液成分是影响微粒体分离和微粒体在两相体系中分配（质膜纯化）的关键因素；排除果肉组织中有机酸、酚类和果胶物质的干扰是确定研磨缓冲液的原则；从设计的８种研磨缓冲液中选出了最佳配方。用最佳研磨缓冲液制备出高得率和高质量的微粒体。两相分配后的标志酶活性检测表明，上相富含质膜，内膜污染较少，质膜纯度较高；而下相富含内膜。证明了ＰＥＧ－Ｄｅｘｔｒａｎ两相分配法对于葡萄果肉组织质膜的纯化也是适用的。
The microsomal and cytoplasmic components were isolated from grape pulp tissue by differential centrifugation, and the microsomes were further separated by two-phase partitioning method. The results showed that the separation of microsomes was a challenge for the whole experimental technique. The composition of grinding buffer was the key factor affecting the separation of microsomes and the distribution of microsomes in the two-phase system (plasma membrane purification). The exclusion of organic acids, phenols and The interference of pectin substances is the principle of determining the grinding buffer; the optimal formula is chosen from the eight grinding buffers designed. The best milling buffer is used to prepare high yields and high quality microsomes. After the two phase distribution of the marker enzyme activity test showed that the upper phase is rich in plasma membrane, less endometrial pollution, plasma membrane purity is higher; while the lower phase is rich in endometrium. It is proved that PEG-Dextran two-phase partitioning method is also suitable for the purification of grape pulp tissue plasma membrane.