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为寻求诱导黄芩愈伤组织的最佳培养条件和激素配比,以黄芩的子叶、真叶和下胚轴为外植体,利用不同激素组合的MS培养基分别在黑暗和光照条件下诱导愈伤组织。结果表明:①在黑暗条件下各激素处理的愈伤组织诱导率和愈伤组织增重均高于光照条件下,而褐化率低于各处理,并且诱导出的愈伤组织较光照下松散,适合于细胞培养;②M6培养基其褐化率为0,诱导率高达100%,愈伤组织增重2.22g,明显优于其它激素配比,是黄芩愈伤组织诱导的最佳激素配比;③各种激素配比处理下,黄芩真叶愈伤组织的愈伤组织增重明显高于子叶和下胚轴。而褐化率却明显低于它们,因此真叶是黄芩诱导愈伤组织的最佳外植体。
In order to seek the best culture condition and hormone ratio for inducing the callus of Scutellaria baicalensis Georgi, the cotyledons, the true leaves and the hypocotyls of Scutellaria baicalensis were used as explants, and MS medium with different hormones was used to induce them under dark and light conditions respectively Wound tissue. The results showed as follows: ① The callus induction rate and callus weight gain of all the hormones under dark conditions were higher than those under the light conditions, while the browning rates were lower than those of the treatments, and the induced callus was more loose than the light , Suitable for cell culture; ②M6 medium browning rate of 0, the induction rate as high as 100%, callus weight 2.22g, significantly better than the other hormone ratio, Scutellaria callus is the best hormone ratio ; ③The callus of callus of Pueraria baicalensis was significantly higher than that of cotyledon and hypocotyls under various hormonal treatments. While the browning rate was significantly lower than them, so true leaf Scutellaria baicalensis callus induction of the best explants.