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建立检测Ｔｈ１／Ｔｈ２相关细胞因子的方法。方法：设计６对用于ＩＬ－２、ＩＬ－４、ＩＬ－１０、ＩＬ－１３、ＩＦＮｒ和β－ａｃｔｉｎ基因扩增的引物，建立可用于Ｔｈ１／Ｔｈ２相关细胞因子检测的ＲＴ－ＰＣＲ技术，同时用切口平移法制备６种细胞因子的ｃＤＮＡ探针，建立ＲＮＡ斑点杂交技术。结果：用ＲＴ－ＰＣＲ和ＲＮＡ斑点杂交技术检测２０种肿瘤细胞株，发现１１／２０为Ｔｈ２型细胞因子表达，８／２０为Ｔｈ０型，１／２０未检测到细胞因子。结论：ＲＴ－ＰＣＲ技术和ＲＮＡ斑点杂交技术是检测Ｔｈ１／Ｔｈ２相关细胞因子的有效方法。
Establish methods to detect Th1/Th2 related cytokines. METHODS: Six pairs of primers for the amplification of IL-2, IL-4, IL-10, IL-13, IFNr, and β-actin genes were designed, and RT-PCR techniques for the detection of Th1/Th2 related cytokines were established. Simultaneously, six kinds of cytokines cDNA probes were prepared by nick translation method, and RNA dot blot hybridization technology was established. RESULTS: Twenty tumor cell lines were detected by RT-PCR and RNA dot blot hybridization. 11/20 were found to be Th2-type cytokines, 8/20 were Th0-type, and 1/20 did not detect cytokines. Conclusion: RT-PCR and RNA dot blotting are effective methods for detecting Th1/Th2 related cytokines.