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目的研究昆明地区2003年9~12月婴幼儿轮状病毒腹泻分子流行病学及临床特点。方法采用逆转录-聚合酶链法(RT-PCR)扩增婴幼儿腹泻大便样本中编码轮状病毒VP7蛋白的全基因片断,用巢式-聚合酶链反应(nested PCR)对扩增得到的VP7基因进行分型。结果1.昆明地区2003年秋冬季50份标本能定型者32份(占64%)。其中G3型占93.75%(30/32);混合型占6.25%(2/32),均为G1+G3型。本次分型中未见G2及G4型。2.2003年9~12月轮状病毒腹泻临床特点为病程短,脱水程度轻,腹泻次数少,电解质紊乱及酸中毒少见。结论1.2003年9~12月昆明地区轮状病毒流行株以G3型为主,与以往比较出现了流行株的转变。2.G1型感染的临床表现较G3型重。
Objective To study the molecular epidemiology and clinical features of infantile rotavirus diarrhea in Kunming from September to December in 2003. Methods The complete genome of rotavirus VP7 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from stool samples of infants with diarrhea. The nested PCR was used to amplify VP7 gene typing. Results 1. Kunming area in autumn and winter 2003 50 specimens can be stereotyped 32 (64%). Among them, G3 accounted for 93.75% (30/32), mixed type accounted for 6.25% (2/32), all of which were G1 + G3. No G2 and G4 were found in this subtype. The clinical features of rotavirus diarrhea from September to December in 2003 are as follows: short course of disease, mild dehydration, few diarrhea, electrolyte imbalance and acidosis. From January 2003 to December 2003, the epidemic strains of rotavirus in Kunming mainly belonged to G3 type, and the transformation of epidemic strains was compared with the previous ones. The clinical manifestations of G1 infection are heavier than that of G3.