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利用ISSR技术对新疆核桃元丰、云新核桃、泰山野核桃和陕西核桃4个核桃实生居群共61份种质进行了遗传多样性分析。结果表明:从36条ISSR引物中筛选9条引物,共检测出101个位点,多态性位点89个,占检测位点总数的88.12%,有效等位基因数、Nei’s基因多样性指数和Shannon信息指数分别为1.5207、0.3125和0.4759。UPGMA聚类结果表明,61份核桃种质聚为5组,元丰核桃居群和陕西核桃首先聚在一起,然后与云新核桃聚在一起,最后与泰山野核桃相聚,其中陕西核桃居群的遗传多样性最高,分为2组,其多态位点百分率、H值和I值分别为66.29%、0.2111和0.3239。居群间遗传一致度在0.6727~0.7970之间,基因流为0.4220,表明不同居群间基因交流程度很低,基因分化明显,在遗传组成上存在显著差异。
ISSR was used to analyze the genetic diversity of 61 germplasms from four walnut populations in Xinjiang, such as Yuanfeng, Yunxin walnut, Taishan wild walnut and Shaanxi walnut. Nineteen primers were screened out from 36 ISSR primers. A total of 101 loci were detected, of which 89 were polymorphic (88.12% of the total). The number of effective alleles, Nei’s gene diversity index And Shannon Information Index were 1.5207, 0.3125 and 0.4759 respectively. UPGMA clustering results showed that 61 walnut germplasm clustered into 5 groups. The population of Yuanfeng walnut and Shaanxi walnut were clustered together first, and then clustered together with Yunxin walnut and finally clustered with Taishan wild walnut, of which walnut population of Shaanxi The genetic diversity was highest in two groups. The percentage of polymorphic loci, H value and I value were 66.29%, 0.2111 and 0.3239, respectively. The genetic identity among populations ranged from 0.6727 to 0.7970, and the gene flow was 0.4220, which indicated that the genetic exchange among different populations was very low and the gene differentiation was obvious with significant differences in genetic composition.