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1原种亲本扩繁田间检验1.1隔离区的检测要检验和测量亲本扩繁田的隔离条件。要求扩繁田周边水平隔离500m以上,否则,视为亲本扩繁田间检验隔离区不合格。1.2苗期原种亲本扩繁田间检验苗期田间检验的方法是,当扩繁原种亲本田间幼苗二叶期至四叶期,0.67hm2以棋盘式取样法,取5点,每点取20株,调查100株幼苗样品,查其叶鞘、叶片、叶缘、叶型等苗期特征特性,与亲本原原种记录的苗期性状相比对,计算该亲本苗期纯度百分比。
1 Protocols Parents Breeding Field Test 1.1 Isolation Zone Testing To test and measure the isolation conditions of the parent propagation field. Requires expansion of the surrounding area around the level of isolation more than 500m, otherwise, as the parent proliferation field test isolation area unqualified. 1.2 Protoplasm Seedling Propagation at Seedling Field The field test at the seedling stage is that when the seedling of the parental seedling is multiplied from the second to the fourth leaf stage, 0.67hm2 is picked up by checkerboard method and taken at 20 points The seedlings of 100 seedlings were investigated and their seedling characteristics such as leaf sheaths, leaves, leaf margins and leaf types were investigated and compared with the seedling traits recorded by their parental elite. The percentage of seedling purity was calculated.