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目的 :探讨乳房恶性肿瘤组织中端粒酶的表达 ,端粒酶与肿瘤病理行为学的关系以及与其他癌基因和抑癌基因的关系。方法 :应用端粒重复扩增方法对 6 0例乳房良恶性肿瘤组织、配对癌旁组织及非癌组织进行端粒酶活性测定 ,并同时进行 P1 6缺失分析及 C-erbβ-2蛋白表达的检测 ,结合临床病理指标进行分析。结果 :乳腺癌中端粒酶活性表达阳性率达 80 %。在乳癌中 P1 6基因缺失、C-erbβ-2蛋白阳性表达同时 ,端粒酶活性检测分别达 92 .3 %和 91.3 %。结论 :说明端粒酶活性和肿瘤的关系密切 ,可作为乳腺癌诊断与预后判断的辅助指标
Objective: To investigate the expression of telomerase in telomerase, the relationship between telomerase and tumor pathology, and the relationship with other oncogenes and tumor suppressor genes. METHODS: Telomeric repeat amplification method was used to detect telomerase activity in 60 benign and malignant thyroid tumors, paired paraneoplastic tissues, and noncancerous tissues. Simultaneous deletion analysis of P16 and expression of C-erbβ-2 protein were performed. Detection, combined with clinical pathological indicators for analysis. Results: The positive rate of telomerase activity in breast cancer was 80%. In the breast cancer, deletion of P16 gene and positive expression of C-erbbeta-2 protein, the detection of telomerase activity reached 92. 3% and 91.3%, respectively. Conclusion : It shows that the relationship between telomerase activity and tumor is closely related. It can be used as an auxiliary index for diagnosis and prognosis of breast cancer.