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为了探索包含SH-2功能域的蛋白酪氨酸磷酸酶(SH-2-containing protein tyrosine phosphatase1,SHP-1)在IL-4诱导的IL-4受体(IL-4receptor,IL-4R)表达中的作用,用Na3VO4处理野生型(wildtype,WT)实验小鼠的脾脏细胞以及用IL-4刺激可育Motheaten小鼠(viable motheatenmice,mev/mev)的脾脏细胞,并检测IL-4RαmRNA的表达.我们发现IL-4诱导的IL-4RαmRNA表达在经Na3VO4处理后野生型小鼠的脾脏细胞以及用IL-4刺激的mev/mev小鼠的脾脏细胞中降低了.结果表明,IL-4诱导的IL-4RαmRNA表达的降低是由于IL-4R的低水平表达导致的STAT6信号转导缺陷.实验进一步证实,在mev/mev小鼠的脾脏细胞中IL-4Rα蛋白表达的降低是由于细胞组成的改变.在mev/mev小鼠脾脏组织中,表达相对高水平IL-4R的CD4+,CD8+和CD19+的细胞比例显著下降,相反表达低水平IL-4R的Mac-1+和Gr-1+细胞比例明显升高.尽管IL-4R蛋白表达是明显下降的,但在同窝对照小鼠(+/?)和mev/mev小鼠的脾脏细胞中IL-4RαmRNA的表达未发现明显的差异.在B细胞、T细胞和巨噬细胞中也没有显著差异.这提示在巨噬细胞中IL-4R表达细胞类型特异性下调是通过转录后水平的调控来实现的.研究结果提示,在脾脏细胞中SHP-1对于IL-4诱导的IL-4R表达是必需的,并且通过影响血细胞生成而间接地调控相应的功能.
In order to explore the role of SH-2-containing protein tyrosine phosphatase 1 (SHP-1) in the expression of IL-4 induced IL-4 receptor , Spleen cells of wildtype (WT) experimental mice and spleen cells of viable mothenmice (mev / mev) mice stimulated with IL-4 were treated with Na3VO4 and the expression of IL-4Ralpha mRNA was examined . We found that IL-4-induced IL-4Rα mRNA expression was reduced in spleen cells of wild-type mice treated with Na3VO4 and in splenocytes of mev / mev mice stimulated with IL-4. The results show that IL-4 induction Is a deficiency of STAT6 signaling due to the low level expression of IL-4R The experiment further confirmed that the decrease of IL-4Rα protein expression in spleen cells of mev / mev mice was due to the cellular composition of . The proportion of cells expressing CD4 +, CD8 + and CD19 + in relatively high levels of IL-4R was significantly decreased in mev / mev mice spleen compared to the proportion of Mac-1 + and Gr-1 + cells that expressed low levels of IL-4R Was significantly higher than that of the control group (P <0.05) .In spite of the significant decrease of IL-4R protein expression in both littermate control mice (+ /?) And mev / mev No significant differences were found in the expression of IL-4Rα mRNA in murine splenic cells, nor in B cells, T cells and macrophages, suggesting that cell type-specific downregulation of IL-4R-expressing cells in macrophages is achieved by Posttranscriptional level.Studies suggest that SHP-1 is required for IL-4-induced IL-4R expression in spleen cells and regulates its function indirectly by affecting hematopoiesis.