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目的 建立HPLC双波长法测定复方罗布麻片Ⅰ中泛酸钙和氢氯噻嗪的含量.方法 采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),以含0.1％辛烷磺酸钠溶液(用磷酸调节pH值至3.0)-乙腈(96∶4)为流动相A,以甲醇为流动相B,采用梯度洗脱,柱温为35℃,检测波长泛酸钙200 nm、氢氯噻嗪275 nm,流速为1.0mL·min-1,进样量为10 μL.结果 泛酸钙和氢氯噻嗪分别在0.025～0.5 μg(r=0.999 6)和0.16～3.2 μg(r=1.000 0)内线性关系良好;平均加样回收率(n=9)分别为101.2％(RSD=0.47％)和101.6％(RSD=0.49％).结论 本法精密度好,结果准确可靠,适用于该制剂的质量检验分析.“,”OBJECTIVE To establish a dual wavelengths of HPLC method for the determination of calcium pantothenate and hydrochlorothiazide in Compound Kendir Leaves tablet Ⅰ.METHODS The Kromasil C18 (250 mm×4.6 mm,5 μm) was used with the mobile phase A [0.1％ octane sulfonic acid sodium (adjust pH to 3.0 with phosphoric acid)-acetonitrile (96:4)]-methanol as mobile phase B with gradient elution.The column temperature was set as 35 ℃ and the detection wavelength was set at 200 nm for calcium pantothenate and 275 nm for hydrochlorothiazide.The flow rate was 1.0 mL·min-1 and the injection volume was 10 μL.RESULTS The linear range was good within 0.025-0.5 μg (r=0.999 6) for calcium pantothenate,0.16-3.2 μg(r=1.000 0) for hydrochlorothiazide,respectively.The average recoveries(n=9) were 101.2％ (RSD=0.47％) and 101.6％ (RSD=0.49％),respectively.CONCLUSION The method is accurate and reliable for the quality control of this compound preparation.