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目的 探讨川芎嗪拮抗链霉素耳毒性损伤机制.方法 将18只豚鼠随机分为三组:对照组,链霉素组(SM组),川芎嗪+链霉素组(TMP+ SM组).对照组每日腹腔注射生理盐水2.5 ml/kg;SM组每日腹腔注射硫酸链霉素450mg/kg,同时在对侧腹腔注射生理盐水2.5ml/kg;TMP+SM组每日腹腔注射川芎嗪注射液100mg/kg,同时在对侧腹腔注射硫酸链霉素450 mg/kg.各组皆连续用药10d,并于实验前后分别进行听性脑干电位(ABR)检测以监测耳毒发生.10d后处死并制备耳蜗标本,进行耳蜗支持细胞的透射电镜观察.结果 SM组ABR阈值明显高于对照组(P＜0.01),而TMP +SM组ABR阈值明显低于SM组(P＜0.01).SM组Hensen细胞胞膜模糊,表面微绒毛减少,线粒体嵴缺损;内柱细胞细胞核异染色质边集.而TMP+ SM组Hensen细胞和内柱细胞形态学改变较SM组明显减轻.结论 链霉素耳毒性损伤能造成耳蜗支持细胞的形态学改变,而TMP可以拮抗这一改变的发生.“,”Objective To study the mechanism of tetramethypyrazine on anti-streptomycin ototoxicity.Methods 18 guinea pigs were randomly divided into three groups(n=6):control group(normal saline 2.5 ml/kg,ip),streptomycin group(450 mg/kg and normal saline 2.5 ml/kg,ip),tetramethypyrazine+streptomycin group(tetramethypyrazine 100mg/kg and streptomycin 450 mg/kg,ip),all continued for 10 days.ABR threshold was measured to detect the ototoxicity.The ultrastructure of Hensen cells and inner pilar cells was observed with transmission electron microscope.Results ABR threshold value was increased in streptomycin group than in control group(P＜0.01),but lower in tetramethypyrazine+streptomycin group than in streptomycin group(P＜0.01).The morphological changes of Hensen cells and inner pilar cells were significantly less in tetramethypyrazine+streptomycin group than in streptomycin group.Conclusion Tetramethypyrazine can antagonist the morphology change of Hensen cells and inner pilar cells induced by streptomycin.