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A new cyclic peptide, prepatellamide A (1), along with three known cyclic peptides (2)-(4), was isolated from the ascidian Lissoclinum patella. The structure of prepatellamide A was determined from one- and two-dimensional 1H and 13C NMR spectra. The known cyclic peptides were identified as patellamides A (2), B (3) and C (4).