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目的:考察薯蓣皂苷与冰片配伍对大鼠局灶性脑缺血的保护作用。方法:大鼠随机分为假手术组、模型对照组、薯蓣皂苷和冰片配伍处方1～6组,其中薯蓣皂苷和冰片配伍按照基线等比增减设计方法,各组均灌胃给药,1次/d,给药体积10 mL.kg-1,连续给药7 d。末次给药后1 h采用线栓法阻塞大脑中动脉制备大鼠局灶性脑缺血模型,大鼠清醒后立刻行为学评分,造模后24 h TTC染色法测定脑梗死面积和脑含水量。结果:与模型对照组比较,处方2,3能降低神经学行为评分、脑梗死面积、脑含水量(P<0.05,P<0.01),处方1能降低脑梗死面积、脑含水量(P<0.05),处方4,5,6无显著性差异。结论:薯蓣皂苷和冰片抗大鼠局灶性脑缺血的最佳配比为8∶2,6∶4。
Objective: To investigate the protection of diosgenin and borneol on focal cerebral ischemia in rats. Methods: The rats were randomly divided into sham operation group, model control group, diosgenin and borneol group prescriptions 1 ~ 6 group, including diosgenin and borneol compatibility according to the baseline ratio of increase or decrease design method, each group were intragastric administration, 1 Times / d, administration volume 10 mL.kg-1, continuous administration for 7 d. One hour after the last administration, occlusion of the middle cerebral artery was performed by occlusion of the middle cerebral artery (MCAO) to establish a focal cerebral ischemia model. The rats were awakened immediately after scalping for behavioral score. TTC staining was used to determine the cerebral infarction area and brain water content . Results: Compared with the model control group, Prescriptions 2 and 3 decreased neurological score, cerebral infarction area and brain water content (P <0.05, P <0.01). Prescription 1 reduced cerebral infarction area and brain water content (P < 0.05), prescriptions 4,5,6 no significant difference. Conclusion: The optimal ratio of diosgenin and borneol to focal cerebral ischemia in rats is 8: 2 and 6: 4.