论文部分内容阅读
【目的】调查黑龙江省大豆灰斑病菌(Cercospora sojina)的生理小种类型,分析黑龙江省大豆灰斑病菌各生理小种间的亲缘关系。【方法】2006—2011年,对黑龙江省进行大豆灰斑病样本的采集和病菌的分离。利用11对SSR引物对黑龙江省的24个灰斑病菌菌株进行EST-SSR基因型分析。【结果】共分离获得大豆灰斑病菌2 674株,鉴定出1—15号共15个生理小种。SSR结果共检测出等位变异46个,平均每个位点为4.2个,相似系数范围为0.091—0.956,平均相似性系数达到0.589。聚类分析结果与大豆灰斑病菌小种类型呈现出较高的相关性。【结论】黑龙江省大豆灰斑病菌间具有丰富的遗传多样性。大豆灰斑病菌的亲缘关系与生理小种类型密切相关,与采集年份、采集地点相关性不大。
【Objective】 The objective of this study was to investigate the physiological races of Cercospora sojina in Heilongjiang Province, and to analyze the genetic relationship among various physiological races of Cercospora sojina in Heilongjiang Province. 【Method】 From 2006 to 2011, the samples of soybean gray leaf spot were collected and the bacteria were isolated from Heilongjiang Province. The EST-SSR genotypes of 24 Cercospora sojina strains in Heilongjiang Province were analyzed using 11 pairs of SSR primers. 【Result】 A total of 2 674 gray spot pathogens were isolated and identified 15 physiological races from 1 to 15. SSR results detected a total of 46 alleles, with an average of 4.2 loci per locus. The similarities ranged from 0.091 to 0.956, and the average similarity coefficient reached 0.589. The cluster analysis showed a high correlation with the races of Cercospora sojina. 【Conclusion】 There is a rich genetic diversity among Soybean gray spot pathogens in Heilongjiang Province. The phylogenetic relationship of Cercospora sojina was closely related to the ecotypes of Physiological races, but not to the collection year and collection site.