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以内蒙古河套灌区湿地植物芦苇(Phragmites australis)根际土壤为材料,采用分离培养法对天然湿地和退化湿地的芦苇根际细菌多样性进行了研究。16SrRNA基因系统发育分析的结果显示,分离自天然湿地芦苇根际的45株细菌分属于γ-变形菌纲(44.44%)、厚壁菌门(Firmicutes)(42.22%)和放线菌门(Actinobacteria)(13.33%)3大类群,其中,γ-变形菌纲为最优势类群,芽孢杆菌属(Bacillus)(42.2%)为最优势菌属;分离自退化湿地芦苇根际的44株细菌分属γ-变形菌纲(13.63%)、β-变形菌纲(6.82%)、厚壁菌门(47.73%)及放线菌门(Actino-bacteria)(31.82%)4大类群,其中厚壁菌门为最优势类群,芽孢杆菌属(40.9%)为最优势菌属。研究结果表明,天然湿地和退化湿地芦苇根际细菌都具有较丰富的多样性,但天然湿地与退化湿地芦苇根际细菌群落结构存在一定差异。
The rhizosphere bacteria diversity of Phragmites australis in Hetao irrigated area of Inner Mongolia was studied by means of isolation and culture method in natural and degraded wetlands. Phylogenetic analysis of 16S rRNA gene revealed that 45 strains of bacteria isolated from the rhizosphere of natural reeds belonged to γ-Proteobacteria (44.44%), Firmicutes (42.22%) and Actinobacteria ) (13.33%). Among them, γ-Proteobacteria was the most dominant group, while Bacillus (42.2%) was the dominant species; 44 strains isolated from the rhizosphere of degraded wetland belonged to genus (13.63%), β-Proteobacteria (6.82%), Firmicutes (47.73%) and Actino-bacteria (31.82%). Among them, Door is the most dominant group, while Bacillus (40.9%) is the most dominant genus. The results showed that rhizosphere bacteria in both natural wetland and degraded wetland were rich in diversity, but there were some differences in the rhizosphere bacterial community between natural wetland and degraded wetland.