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目的测定长角血蜱(Haemaphysalis longicornis)感染汉赛巴尔通体(Bartonella henselae)后不同时间段超氧化物歧化酶的活性。方法用Hamilton 10μL微量进液器将汉赛巴尔通体菌液悬浮液(浓度为105cfu/μL),通过假头与盾板间缘凹处注入已部分吸血的未交配的长角血蜱成蜱血腔内,在染毒后1 h,6 h,12 h,24 h及48 h后收集长角血蜱的血淋巴,使用南京建成超氧化物歧化酶(SOD)试剂盒测定其血淋巴中SOD的活性。同时设立生理盐水对照组,对比两组之间及两组内各时间点是否存在差异。结果对收集到的不同时间段的血淋巴测定超氧化物岐化酶的活性后发现,实验组在感染后48 h内,酶活性一直处在平稳的水平;生理盐水对照组SOD酶活性随着时间的延长先升高后又降低,在12 h达到(11.1999±1.3248)U/mL,并且与实验组此时间段的酶活性相比有统计学差异(P=0.036);而在48 h降为最低,与1 h的酶活性相比有统计学差异(P=0.000)。结论长角血蜱受到一定浓度的汉赛巴尔通体攻击后,血淋巴SOD酶活性在一段时间内受到了抑制。
Objective To determine the activity of superoxide dismutase (SOD) at different time points after infection of Bartonella henselae by Haemaphysalis longicornis. Methods The Hamilton suspension (concentration: 105 cfu / μL) was inoculated into the suspension of ticks through the concave edge between the fake head and the shield plate. The haemolymph of Haemaphysalis gondii collected at 1, 6, 12, 24 and 48 h after exposure were assayed for the level of SOD in haemolymph by using the kit of Nanjing superoxide dismutase (SOD) Activity. At the same time set up saline control group, comparing the two groups and the two groups within each time point whether there are differences. Results The activities of superoxide dismutase (SOD) in haemolymph collected in different time periods were determined. The results showed that the activity of SOD in the experimental group was stable at 48 h after infection. The activity of SOD in the control group The prolongation of time increased first and then decreased and reached (11.1999 ± 1.3248) U / mL at 12 h, which was significantly different from that of experimental group (P = 0.036) at 48 h Which is the lowest, which is statistically different from that of 1 h (P = 0.000). Conclusions The hemolymph activity of SOD was inhibited for some time after being attacked by certain concentration of Bartonella henselae.