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目的探讨α-内收蛋白(ADD1)基因 G460W 和 G 蛋白β3亚单位(GNB3)基因 C825T多态与高血压的关系。方法运用多聚酶链式反应-限制性内切酶片段长度多态分析方法(PCR-RFLP)对来自山东省青岛港的正常血压(495例)和高血压(256例)人群进行基因分型。结果高血压组和对照组 ADD1基因 G460W 和 GNB3基因 C825T 多态型及等位基因频率分布均符合 Hardy-Weinberg 平衡。高血压患者中具有 WW 基因型个体的频率(35.2%)高于对照组(27.4%),差异有统计学意义(X~2=4.768,P=0.029,OR=1.43)。在高血压组和对照组中 GNB3基因 CC、CT、TT 基因型频率和 C、T 等位基因频率差异无统计学意义(基因型,P=0.755;等位基因,P=0.561)。结论ADD1基因 G460W 多态可能是高血压的危险因素,460W 等位基因可能与收缩压的增加有关,而GNB3基因 C825T 多态可能在高血压的发生中不起主要作用。
Objective To investigate the relationship between C825T polymorphism of G460W and GNB3 gene in hypertension and hypertension. Methods The genotypes of normal blood pressure (495 cases) and hypertension (256 cases) from Qingdao port in Shandong province were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The polymorphism of C825T polymorphism and allele of G460W and GNB3 in ADD1 gene in hypertensive group and control group all accord with Hardy-Weinberg equilibrium. The frequency of individuals with WW genotype in hypertensive patients was higher than that in controls (35.2% vs 27.4%, X 2 = 4.768, P = 0.029, OR = 1.43). There were no significant differences in the frequencies of genotypes CC, CT, TT and C, T alleles between the hypertensive group and the control group (genotype, P = 0.755; allele, P = 0.561). Conclusion G460W polymorphism of ADD1 gene may be a risk factor for hypertension. The 460W allele may be related to the increase of systolic blood pressure. However, the C825T polymorphism of GNB3 gene may not play a major role in the development of hypertension.