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目的建立检测环境水体中嗜肺军团菌聚合酶链(PCR)法。方法以嗜肺军团菌特异性保守基因Mip为靶基因,采用Godkex软件设计引物,聚合酶链反应测定。结果该方法检测军团菌的灵敏度为5.8×102cfu/ml,6株标准军团菌均扩增出996 bp的条带,4株非嗜肺军团菌和4株非军团菌无条带出现;检测71份环境水样,5份阳性,阳性率为7.0%。结论该方法快速、灵敏、特异,是检测水体中嗜肺军团菌的有效方法。
Objective To establish a polymerase chain reaction (PCR) method for detecting Legionella pneumophila in environmental water. Methods Legionella pneumophila specific conserved gene Mip was used as a target gene. Godkex software was used to design primers and polymerase chain reaction (PCR). Results The sensitivity of this method to detect Legionella was 5.8 × 102cfu / ml. The results showed that 996 bp bands were amplified by 6 standard Legionella strains, 4 bands of non-Legionella pneumophila and 4 strains of non-Legionella were detected. Detection 71 A copy of the environmental water samples, 5 were positive, the positive rate was 7.0%. Conclusion The method is rapid, sensitive and specific and is an effective method to detect Legionella pneumophila in water.