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本研究以一年生黑麦草(Lolium multiflorum L.)为材料,通过农杆菌介导的茎尖转化法将降解除草剂2,4-D的Tfd A基因转入黑麦草。除草剂2,4-D喷施实验表明野生型黑麦草在10 g/L 2,4-D喷施17 d后黄化死亡,而转基因植株(转Act1::Tfd A基因和Act1::Tfd A-Zml3::Flp基因)在10 g/L 2,4-D处理下仍生长良好,喷施10 g/L 2,4-D后所有植株叶片的叶绿素含量均下降,分别为2.411 mg·g-1·FW、3.006 mg·g-1·FW和3.039 mg·g-1·FW,比喷施前下降了24.444%、2.592%和1.619%。Tfd A基因在黑麦草中的表达显著提高了转Tfd A基因黑麦草抗2,4-D除草剂的能力。
In this study, annual Lolium multiflorum L. was used as the material to transfer the Tfd A gene from 2,4-D into ryegrass via agrobacterium-mediated shoot-tip transformation. The herbicide 2,4-D spraying experiments showed that the wild-type ryegrass died after 17 days spraying with 10 g / L 2,4-D, while the transgenic plants (Act1 :: Tfd A gene and Act1 :: Tfd A-Zml3 :: Flp gene) grew well under 10 g / L 2,4-D treatment. Chlorophyll content of all plant leaves decreased after spraying 10 g / L 2,4-D, which were 2.411 mg · g-1 · FW, 3.006 mg · g-1 · FW and 3.039 mg · g-1 · FW, decreased by 24.444%, 2.592% and 1.619%, respectively. The expression of Tfd A gene in ryegrass significantly enhanced the ability of transgenic ryegrass to resist 2,4-D herbicide.