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本文对香菇（Ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ）单核菌丝９１０１（１２）原生质体制备后的再生条件，进行了探索。结果表明，原生质体经过合０．６Ｍ．甘露醇的麦芽糖酵母粉培养基（ＭＹＧ）液体培养５天，用双层培养法，在含０．６ＭＭｇＳＯ４的高渗ＭＹＧ平板上经再生，再生率达５×１０－５。本文将Ｋｎｏｗｌｔｏｎ等于１９８４年创造的一种分离高频再生衍生株的方法首次运用至食用菌中，所得９１０１（１２）再生株Ｒ３，再生率提高２～３倍，为香菇原生质体融合操作打下基础。
In this paper, the regeneration conditions of protoplasts of Lentinousodes monokaryon 9101 (12) were explored. The results showed that the protoplasts through the combined 0.6M. The mannitol maltose yeast culture medium (MYG) liquid was cultured for 5 days, and was regenerated on a hypertrophic MYG plate containing 0.6MMgSO4 by a double layer culture method at a regeneration rate of 5 × 10-5. In this paper, a method of separating high frequency regenerated derivative strains, which is created by Knowlton et al. In 1984, was first applied to edible fungi. The obtained 9101 (12) regenerated strain R3 improved the regeneration rate by 2 to 3 times, laying a foundation for the protoplast fusion of mushroom .