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试验采用武汉市场上来源不同产地的 7份马铃薯材料 ,用CIP提供的RS NCM ELISA试剂盒进行了块茎青枯病菌携带情况的检测。试验结果显示 ,应用该试剂检测马铃薯块茎青枯病菌携带情况具有较高的灵敏度 ;检测前样品的富集时间对检测结果具有明显的影响 ,其最佳富集时间为4 8h ;不同来源的马铃薯块茎携带青枯病菌的程度具有显著差异
Seven potato materials from different sources in Wuhan market were used in this experiment. The detection of carriage of R. solanacearum by RS NCM ELISA kit provided by CIP was carried out. The results showed that the detection of potato tuberous bacterial wilt with this reagent had a high sensitivity. The enrichment time of pre-test samples had a significant effect on the test results, and the optimum enrichment time was 48 h. Potatoes from different sources The extent of tuberous bacterial wilt disease was significantly different