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目的制备一种载金纳米棒(Au)和液态氟碳全氟己烷(PFH)的纳米级造影剂,并对其进行体外双模态(光声/超声)显影研究,进一步实现体内显影。方法采用双乳化法制备包裹金纳米棒-液态氟碳的高分子聚合物(PLGA)纳米粒(GNPs)及只包裹双蒸水的纳米粒(WNPs)或只包裹金纳米棒的纳米粒(Au-NPs);MTT对不同浓度的GNPs行体外细胞毒性实验;分别对GNPs、W-NPs、A-NPs在激光辐照前后二维超声、造影强度和光声信号显影,并统计激光辐照前后二维超声灰度值、造影值及光声信号值。结果成功制备包裹金纳米棒-液态氟碳双模态纳米级造影剂,体外光声和造影效果好;GNPs对HUVECs的毒性,各个浓度抑制率较低,且各组间无统计学差异;在激光辐照前,GNPs、Au-NPs、W-NPs二维超声及造影模式下均为低回声;激光辐照后,GNPs超声灰度和造影强度明显增强,光声显影明显,W-NPs超声灰度和造影模式未见增强,光声未见显影,Au-NPs超声灰度造影强度未见明显增强,光声显影明显。结论成功制备包裹金纳米棒-液态氟碳的纳米级造影剂,实现了体外超声/光声双模态增强显影,为体内靶向显影提供实验基础。
OBJECTIVE To prepare a nanoscale contrast medium loaded with gold nanorods (Au) and liquid fluorocarbon perfluorohexane (PFH), and to conduct in vitro bimodal (photoacoustic / ultrasonic) imaging studies for further in vivo imaging. Methods The double-emulsion method was used to fabricate gold nanoparticles (GNPs) coated with gold nanorods - liquid fluorocarbons and nanoparticles coated with double distilled water (WNPs) or gold nanorods -NPs); MTT cytotoxicity in vitro to different concentrations of GNPs; Two-dimensional ultrasound, contrast intensity and photoacoustic signal imaging of GNPs, W-NPs and A-NPs were performed before and after laser irradiation respectively. Dimensional ultrasound gray value, contrast value and photoacoustic signal value. Results The gold nanorods-liquid fluorocarbon dual-mode nano-scale contrast agent was successfully prepared. The in vivo photoacoustic and contrast effects of GNPs were good. The toxicity of GNPs to HUVECs was low, with no significant difference among the groups. There was no significant difference between the groups Before laser irradiation, the GNPs, Au-NPs and W-NPs were both hypoechoic under two-dimensional ultrasound and contrast mode. After laser irradiation, the gray scale and contrast intensity of GNPs were significantly enhanced, and the photoacoustic imaging was obvious. Grayscale and contrast mode did not increase, no visualization of photoacoustic imaging, Au-NPs ultrasound grayscale imaging intensity was not significantly enhanced, photoacoustic imaging significantly. Conclusion The nano-scale contrast agent coated with gold nanorods and liquid fluorocarbons has been successfully prepared, and the ultrasound / photoacoustic dual-mode enhanced imaging can be achieved in vitro, providing the experimental basis for targeted development in vivo.