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目的 研究中国版纳小型猪心脏的异种抗原α1,3 Gal的分布和半定量分析 ,为小型猪心脏的转基因和异种移植提供资料。方法 通过亲和免疫组织化学法和图像分析对 10头版纳小型猪心脏进行α1,3 Gal的分布和半定量研究。结果 α1,3 Gal主要分布于细胞膜 ,细胞浆也有少量表达 ,胞核无表达。在小型猪心内膜、心肌间质、心外膜、神经束膜等处有α1,3 Gal分布 ,心肌细胞未发现α1,3 Gal分布。半定量分析发现α1,3 Gal表达量心房内膜最多 ,心肌间质最少。结论 异种抗原α1,3 Gal在心脏中的表达存在较大差异 ,提示可以通过转基因猪来克服超急性异种排斥反应
Objective To study the distribution and semi-quantitative analysis of the heterologous antigen α1,3 Gal in the heart of Chinese miniature piglets and provide information on the transgene and xenotransplantation of miniature piglets. Methods The distribution and semi-quantitative studies of α1,3 Gal in 10 Banna miniature pig hearts were performed by affinity immunohistochemistry and image analysis. Results α1,3 Gal mainly distributed in the cell membrane, cytoplasm was also a small amount of expression, no expression in the nucleus. In pigs endocardium, myocardial interstitial, epicardial, perineurium and other places have α1,3 Gal distribution, myocardial cells found no α1,3 Gal distribution. Semiquantitative analysis found α1,3 Gal expression in the most atrial intima, myocardial interstitium at least. Conclusion The expression of heterologous antigen α1,3 Gal in the heart is quite different, suggesting that hypersensitive xenograft rejection can be overcome by transgenic pigs