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目的:构建兔下颌前牙即刻种植后种植体周围炎动物模型。方法:选取12只健康雄性新西兰大白兔,麻醉后拔除兔下颌左侧前牙,在拔牙窝中即刻植入锥柱状纯钛种植体。待种植体植入8周后,随机选择3只实验兔处死,采用Micro-CT观察种植体骨结合状况。剩余实验兔随机分为3组:空白组不加刺激,对照组种植体周围注射磷酸盐缓冲溶液,实验组注射细菌内毒素溶液。加刺激2周后处死实验动物,取含种植体的下颌骨标本,采用Micro-CT观测骨量;取种植体周围牙龈标本,采用HE染色,观察牙龈组织病理学改变。结果:即刻种植8周后,种植体骨结合良好,牙龈健康。加刺激2周后,空白组和对照组牙龈健康呈粉红色,质地坚韧,种植体骨结合良好,种植体顶部骨组织无明显吸收,牙龈组织中见散在炎症细胞;实验组牙龈红肿,质地软,种植体顶部牙槽骨明显吸收,骨密度和骨体积分数显著降低,牙龈组织中见大量炎症细胞弥散性浸润。结论:种植体周围局部注射细菌内毒素可快速构建兔种植体周围炎原位动物模型。
Objective: To construct an animal model of peri-implant inflammation after immediate implantation of the mandible anterior teeth. Methods: Twelve healthy male New Zealand white rabbits were selected and the left mandibular anterior teeth were removed after anesthesia. Conical columnar titanium implants were implanted immediately in the extraction socket. After the implants were implanted for 8 weeks, three rabbits were randomly selected and sacrificed. Micro-CT was used to observe the osseointegration. The remaining experimental rabbits were randomly divided into three groups: the blank group without stimulation, the control group injected around the implant phosphate buffer solution, the experimental group injected bacterial endotoxin solution. After 2 weeks of stimulation, the experimental animals were sacrificed and the mandibular specimens containing the implants were taken. The bone mass was observed by Micro-CT. The gingival samples around the implants were taken and the pathological changes of gingival tissues were observed by HE staining. Results: After 8 weeks implants, the implants were well bonded and the gums were healthy. After 2 weeks of stimulation, the gingival health of the blank group and the control group was pink, the texture was tough, the implant bone was well bonded, the bone tissue at the top of the implant did not absorb obviously, and the inflammatory cells were scattered in the gingival tissue. , The alveolar bone on the top of the implant was obviously absorbed, the BMD and the volume fraction of the bone were significantly reduced, and a large number of diffuse infiltration of inflammatory cells were seen in the gingival tissue. Conclusion: Local injection of bacterial endotoxin around implants can rapidly establish an animal model of peri-implantitis in rabbits.